The preparation of the Instant Cells will take less than 15 minutes. No further splitting or cultivation procedure is needed. The protocol varies a bit from platform to platform. The preparation procedure is roughly outlined in the following: Take one cap containing Instant Cells out of the liquid nitrogen container, screw it on a 15 ml tube containing extracellular buffer, shake the cells smoothly for 2 minutes, spin down, wash and resuspend the pellet in extracellular buffer.
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