The Instant Cells Kit includes:

• Caps with stably transfected Na_v1.5 CHO cells
  (formerly: SCN5A, SKM2).
• Intracellular solution.
• Extracellular solution.
• QA certificate.
• Cell Reservoir.

Na_v1.5 electrophysiology

CHO Instant Cells expressing the human cardiac Na_v1.5 sodium channel were characterized regarding electrophysiological and pharmacological properties using conventional whole cell recordings.

Representative current traces were recorded from Na_v1.5-CHO Instant Cells. Typical currents were evoked by a voltage-step protocol starting from a holding potential of -100 mV in 10 ms pulses to test voltages from -60 to +80 mV in 10 mV increments. The current-voltage relationship of the Instant Cells are virtually identical to that observed with a running cell culture.

Na_v1.5 pharmacology

Dose-response relationships for different sodium channel blockers were obtained in whole-cell patch clamp recordings.

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