CHO Nav1.5 INSTANT CELLS
The Instant Cells Kit includes:
- Caps with stably transfected Nav1.5 CHO cells
(formerly: SCN5A, SKM2). - Intracellular solution.
- Extracellular solution.
- QA certificate.
- Cell Reservoir.
Nav1.5 electrophysiology
CHO Instant Cells expressing the human cardiac Nav1.5 sodium channel were characterized regarding electrophysiological and pharmacological properties using conventional whole cell recordings.
Representative current traces were recorded from Nav1.5-CHO Instant Cells. Typical currents were evoked by a voltage-step protocol starting from a holding potential of -100 mV in 10 ms pulses to test voltages from -60 to +80 mV in 10 mV increments. The current-voltage relationship of the Instant Cells are virtually identical to that observed with a running cell culture.
Nav1.5 pharmacology
Dose-response relationships for different sodium channel blockers were obtained in whole-cell patch clamp recordings.
| Compound | IC50: Instant Cells | IC50: running cell culture |
|---|---|---|
| TTX | 2.22 µM | 2.18 µM |
| Flecainide | 11.99 µM | 14.81 µM |
| Mexilentine | 20.77 µM | 22.53 µM |
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CONTACT
Cytocentrics Bioscience GmbH
Phone: +49(0)381 440 388-0
E-Mail: info@cytocentrics.com
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