hERG HEK 293 INSTANT CELLS

The Instant Cells Kit includes:

• Caps with stably transfected hERG-HEK 293 cells
• Intracellular solution
• Extracellular solution
• QA certificate
• Cell Reservoir

hERG electrophysiology

Instant HEK 293 cells expressing the human ERG (Ether-a-go-go Related Gene) potassium channel were characterized regarding their electrophysiological and pharmacological properties using conventional whole cell recordings. hERG currents were activated by depolarizing voltage steps beginning at –50 mV. Further depolarization led to a decrease in outward current amplitudes due to the onset of rapid inactivation.

Representative hERG outward currents were recorded from Instant HEK 293 cells stably expressing the hERG ion channel. hERG Currents were elicited upon depolarization of the membrane to +40 mV in 10 mV increments. hERG tail currents were elicited by means of a repolarizing step to -50 mV (2 s).

hERG pharmacology

Dose response data for different compounds were obtained with conventional patch clamp. After whole cell establishment, increasing concentrations of quinidine, cisapride, E4031 and terfenadine were applied to the cells.