hERG STUDY WITH CYTOPATCH™

CytoPatch™ study: Effect of Cisapride, (d-l)-Sotalol and Terfenadine on hERG channel currents of transfected CHO cells

In this study, the operating efficiency of the CytoPatch™ instrument was compared to conventional patch clamping. We investigated the effect of Cisapride, (d-l)-Sotalol and Terfenadine on the hERG channel in transfected CHO cells.

There was a good agreement between IC50 values obtained with conventional patch clamping and IC50 values obtained with the CytoPatch™ instrument (values for conventional Patch-Clamping/ CytoPatch™: Cisapride 6,2/ 8,6 nM, (d-l)-Sotalol 281/ 361 µM, Terfenadine 8,4 / 29,6 nM).

When compared to values from the literature, our obtained data are clustered in the lower range. This reflects the high quality of the data generation and the good performance of the micro fluidic compound application.

A detailed analysis of the efficiency (with a total success rate of 75%) as well as the seal rate (median of seal resistance is 2.26 GOhm) was evaluated with Cisapride as an example.

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